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KMID : 0043320160390010059
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Archives of Pharmacal Research
2016 Volume.39 No. 1 p.59 ~ p.65
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Measuring levels of biogenic amines and their metabolites in rat brain tissue using high-performance liquid chromatography with photodiode array detection
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Gu Min-Jung
Jeon Ji-Hyun
Oh Myung-Sook
Hong Seon-Pyo
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Abstract
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We developed a method to detect biogenic amines and their metabolites in rat brain tissue using simultaneous high-performance liquid chromatography and a photodiode array detection. Measurements were made using a Hypersil Gold C-18 column (250 × 2.1 mm, 5 μm). The mobile phase was 5 mM perchloric acid containing 5 % acetonitrile. The correlation coefficient was 0.9995?0.9999. LODs (S/N = 3) and LOQs (S/N = 10) were as follows: dopamine 0.4 and 1.3 pg, 3, 4-dihydroxyphenylacetic acid 8.4 and 28.0 pg, serotonin 0.4 and 1.3 pg, 5-hydroxyindolacetic acid 3.4 and 11.3 pg, and homovanillic acid 8.4 and 28.0 pg. This method does not require derivatization steps, and is more sensitive than the widely used HPLC?UV method.
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KEYWORD
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Dopamine, Serotonin, Simultaneous determination, Brain tissue, HPLC?PDA, Direct method
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